Purification and Characterization of an Extracellular Protease from Xenorhabdus nematophila Involved in Insect Immunosuppression
نویسندگان
چکیده
منابع مشابه
Purification and characterization of an extracellular protease from Xenorhabdus nematophila involved in insect immunosuppression.
Xenorhabdus nematophila, a bacterium pathogenic for insects associated with the nematode Steinernema carpocapsae, releases high quantities of proteases, which may participate in the virulence against insects. Zymogram assays and cross-reactions of antibodies suggested that two distinct proteases were present. The major one, protease II, was purified and shown to have a molecular mass of 60 kDa ...
متن کاملCharacterization of an Extracellular Protease from the Insect Pathogen Xenorhabdus luminescens.
Xenorhabdus luminescens Hm cultured in gelatin broth produced a single extracellular protease. The protease was purified by a factor of 500 and characterized as a monomeric protein with an approximate molecular weight of 61,000. On the basis of inhibitor studies and its pH optimum, the protease was classified as an alkaline metalloprotease with a pH optimum near 8; the isoelectric point of the ...
متن کاملInsecticidal pilin subunit from the insect pathogen Xenorhabdus nematophila.
Xenorhabdus nematophila is an insect pathogen and produces protein toxins which kill the larval host. Previously, we characterized an orally toxic, large, outer membrane-associated protein complex from the culture medium of X. nematophila. Here, we describe the cloning, expression, and characterization of a 17-kDa pilin subunit of X. nematophila isolated from that protein complex. The gene was ...
متن کاملXenorhabdus nematophila: Mutualist and Pathogen
T he gram-negative bacterium Xenorhabdus nematophila engages in a mutualistic relationship with a specific soil nematode and also can mount potent pathogenic attacks on a variety of insects. During its mutualisticpathogenic life cycle, Xenorhabdus produces a wide range of exoenzymes, antimicrobial and nematicidal compounds, and insect toxins. Moreover, the nematode-bacteria complex is useful fo...
متن کاملPURIFICATION AND PROPERTIES OF AN EXTRACELLULAR PROTEASE PRODUCED BY PENICIUIUM EXPANSUM
Penicilliurn expamum grown in a medium with rice husk as a carbon source produced an extracellular protease. The protease enzyme was isolated from culture broth by fractionation with acetone and column chromatography on Sephadex G- 100 and DEAE A-50. The protease enzyme was purified about 17.47 fold, with a recovery of 14%. The purified protease was homogenous on SDS polyacrylarnide disc ge...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2002
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.68.3.1297-1304.2002